At the 2016 ASMS annual meeting in San Antonio, the Goodlett lab will have the following 12 posters and 1 oral presentation. Check out the links below to download posters from our lab. The conference details may be found by searching at for "Goodlett" on the conference planner function here.
- Scott et al. Absorption mode processing of MALDI-FT-ICR imaging data improves mapping of Gram-negative bacterial virulence factors on-tissue
- Yoon et al. Bio-molecule characterization using a novel ion mobility orbitrap mass spectrometer [pdf]
- Kilgour et al. Autopiquer – introducing a new approach to high confidence peak detection
- Goo et al. Top-down mass spectrometry applications for detection of N-terminal sequence heterogeneity and PTMs for a therapeutic molecule
- Awasthi et al. Phosphoproteomic analysis of differential protein expression in BRaf-Mutated melanoma cells with acquired resistance to BRaf, MEK1/2, or ERK1/2 inhibitors
- Oyler et al. Structural characterization of membrane glycolipids from marine sponge-associated bacteria by mass spectrometry [pdf]
- Khan et al. Top-down Structural elucidation of gram-negative bacterial endotoxins by tandem mass spectrometry [pdf]
- Leung et al. Structural characterization of lipid biomarkers fromStaphylococcus aureusfollowing microextraction for mass spectrometric phenotyping [pdf]
- Zambrzycki et al. Surface Acoustic Wave Nebulization Sample Introduction for Vacuum-Assisted Plasma Ionization
- Yen et al. Surface Acoustic Wave Nebulization – Mass Spectrometry: A Tool for Rapid Analysis of Food Products
- Liang et al. Performance Characterization of Surface Acoustic Wave Nebulization for Lipid A Mass Spectrometric Analysis [pdf]
- Goodlett et al. Surface Acoustic Wave Nebulization – Mass Spectrometry on a TripleTOF Mass Spectrometer
- Chandler et al. A Rapid, Cell Culture-Based Method for Biomarker Discovery and Drug Screening by MALDI-MSI
In preparation for the celebration of MSBM X a new website has been designed by the folk at the University of Amsterdam. Please check it out here www.msbm.org. Thanks to the Corthals Lab for setting this up!
Read more about it here.
Dave and Maureen celebrate the Waters COI program at Charleston in Baltimore, MD during ASMS 2014.Click to read Water’s interview of Dave and Maureen.
SAWN on the SYNAPT G2S with the LC door swung back on the gas off
Tekes and the Academy of Finland, who are sponsors of the Finland Distinguished Professor (FiDiPro) program that Dave is participating in, recently began making so-called FiDiPro video portraits of all of the participants. This one chronicles Dave and the diabetes and ovarian cancer projects where they are looking for protein biomarkers of disease using previously published methods from the Goodlett laboratory; namely a label free method for profiling large numbers of samples (Foss et al. Nat Gen 2007) and a deep sequencing method known as PAcIFIC (Panchaud et al. Anal Chem 2009) both of which have been transferred to Turku. These two methods are being coupled with the accurate mass and time (AMT) tag approach for profiling several hundred clinical samples in 2014 and 2015 et al.Biotechniques. 2004).
- Edgar et al. "Coupling Surface Acoustic Wave Nebulization with Droplet Microfluidics for Rapid Hydrogen Deuterium Exchange Mass Spectrometry"
- Goo et al. "Proteomic discovery and validation on a single MS platform using a PAcIFIC directed pseudo-SRM"[pdf]
- Yen et al. "PDMS Microchannels Coupled to Surface Acoustic Wave Nebulization Mass Spectrometry"
- Heron et al. "Optimizing Sensitivity of Surface Acoustic Wave Nebulization for MS"[pdf]
- Dennison et al. "Development and Application of Surface Acoustic Wave Nebulisation for Top-Down Protein Fragmentation"
- Monkkonen et al. "Native Mass Spectrometry of Noncovalent Protein Complexes by Surface Acoustic Wave Nebulization"[pdf]
- Yoon et al. "Surface Acoustic Wave Nebulization Mass Spectrometry Facilitates Lipid A Analysis"[pdf]
- Jones et al. "Repair of the Yersinia pestis Palmitoyl Transferase (PagP) Restores a Robust LPS Pro-Inflammatory Response"[pdf]
- Kao et al. "Probabilistic E-value Scoring for the Precursor Ion Independent Top-down Algorithm"[pdf]
- Masselon et al. "Surface acoustic wave nebulization mass spectrometry for rapid and sensitive amino acid analysis"[pdf]
- Pan et al. "Mass spectrometry based quantitative proteomics to investigate plasma proteome alterations associated with pancreatic cancer"
- Liu et al. "Protein Identification Using Top-Down Spectra"
- Huang et al. "Defining Limit of Detection of Surface Acoustic Wave Nebulization (SAWN) MS"[pdf]
- Chapman et al. "A novel proteomics approach to quantitative direct sample infusion: rapid protein identification and quantification using data-independent acquisition"
- Huang et al. "Comparison of internal energy deposition with Surface Acoustic Wave Nebulization (SAWN) and Electospray Ionization"
- Jung et al. "Derivation of five essential factors to predict peptide tandem mass spectrometric detectability"
- Monkkonen et al. "Tandem MS analysis of peptides proteolyzed on and nebulized from a surface acoustic wave chip"
- Nguyen et al. "Top-down Precursor Acquisition Independent From Ion Count (PAcIFIC)"
- Singh et al. "Mass spectrometric strategies for improved identification of cross-linked peptides"
- Ting et al. "Cross correlation score aids objective Lipid A structure assignment"
- To et al. "Profiling of bioactive chocolate extracts by high resolution mass spectrometry"
- Yoon et al "Structural analysis of Lipid A using Surface Acoustic Wave Nebulization"
The SAWN method provides a new way to introduce nonvolatile samples into a mass spectrometer without the need for capillaries or any nozzle as is needed by electrospray ionization, while maintaining the benefits of multiple charging. These features will accelerate lipid structure analysis carried out by the lab.