The web page of the Third International UPPSALA Conference on Electron Capture and Transfer Dissociation in Mass Spectrometry: Fundamentals and Applications, which the lab hosted in 2005. It is kept here as a record, worded as it was before the meeting; the email addresses and the links it carried have been left out.
Conference
We are hosting the third international meeting on electron capture dissociation (ECD) to include electron transfer dissociation (ETD) and related methods for electron-based fragmentation in mass spectrometers. Lectures will cover both the fundamental physical chemistry (or chemical physics depending on your orientation) of how the process may work as well as applications in biology; e.g. proteomics and glycomics.
6–9 December 2005 · Salish Lodge and Spa, 45 minutes from SeaTac International Airport, downtown Seattle and skiing
History
Two meetings have been held to date. In 2003 the first meeting was hosted by ECD co-inventor Dr Roman Zubarev on a cruise ship sailing from Stockholm, Sweden to Helsinki, Finland. In 2004 Dr Patrick Langridge-Smith hosted the 2nd at the Marine Hotel in North Berwick, Scotland. The 3rd meeting will be hosted by Dr David Goodlett at Salish Lodge and Spa and will include ETD and related methods.
In 2004 some of the lecturers included: Drs Roman Zubarev of Uppsala University, Matthias Mann of University of Southern Denmark, Chris Hendrickson of the National Magnet laboratory, Takashi Baba of Hitachi Ltd, Peter O'Connor of Boston University, Kristina Hakansson of University of Michigan, Robert Hudgins of York University, David Goodlett of University of Washington and Einar Uggerud of University of Oslo.
In 2003 many of the same gave lectures, as well as Dr Fred McLafferty of Cornell and Dr Frank Turecek of University of Washington. Topics have ranged from fundamental physics of electron capture to applications in proteomics and glycomics. Attendance has ranged from 40 in 2004 to 60 in 2003.
Agenda
Attendees will arrive Tuesday afternoon 6 December. Posters will be set up and left up for the duration of the meeting. That evening, after attendees are settled in a reception with light hors d'ouvre an orientation on the spa and a lecture describing the historical use of electrons to fragment molecules in mass spectrometry leading up to ECD will commence. On Wednesday there will be at least 12 lectures followed by poster viewing and a mixer. In the event that there is snow on nearby Snoqualmie Pass, Thursday morning will be open for skiing and general ad hoc discussions. For those not wishing to play in the snow the possibility of hiking around the lodge (which is at low enough elevation that there should be no snow) or experiencing the relaxation of Japanese style spa exists during this time. Six lectures will be presented Thursday afternoon followed by a banquet that evening, and a lecture projecting the future of the field. Depending on the response six more lectures may be held Friday morning with a Friday afternoon departure.
Speakers
To date the following have agreed to participate:
“The odd electrons are back - and life is exciting again!”
Professor Cathy Costello, Boston University School of Medicine, USA
“New Mass Spectrometry Technology and Applications in the Study of Cell Migration, the Histone Code, and Cancer Vaccine Development”
Professor Donald F. Hunt, University of Virginia, USA
“Effects of Molecular Conformation on ECD”
Professor Evan Williams, University of California at Berkeley
“Charge-reduced arginine, lysine, and histidine residues. Structures and energetics”
Professor Frank Turecek, University of Washington, USA
“ECD - Controversy Helps Its Future”
Professor Fred McLafferty, Cornell University, USA
“Electron capture dissociation FT-ICR mass spectrometry of proteins involved in signal transduction”
Dr. Helen J. Cooper, University of Birmingham, UK
“The Electron Transfer Event in Electron Transfer Dissociation”
Professor Jack Simons, University of Utah, USA
“Electron-Promoted Ion Coherence -Enhancement of ECD and LC/MS Sensitivity”
Professor James E. Bruce, Washington State University, USA
Title to be determined
Professor Jasna Peter-Katalinic, University of Münster, Germany
“ECD of Sulfated Glycosaminoglycans - A Tool for Determining the Sites of Sulfation”
Professor Jon Amster, University of Georgia, USA
“Electron Capture Dissociation of Triply Charged Peptides Carrying a Proton and a Divalent Metal Cation”
Professor Kristina Hakansson, University of Michigan, USA
Title to be determined
Dr. Ljiljana Pasa-Tolic, Pacific Northwest National Laboratory, USA
“The Role of Free Radical Rearrangements in Electron Capture Dissociation”
Professor Peter O'Connor, Boston University School of Medicine, USA
“Predicting fragment abundances in ECD: from full understanding to total confusion and back”
Professor Roman Zubarev, Uppsala University, Sweden
“Towards an Understanding of Electron Transfer Dissociation”
Dr. Sharon Pitteri, Purdue University, USA
“Evolution and revolution in ECD FT-ICR MS implementation”
Dr. Yury Tsybin, National High Magnetic Field Laboratory, USA
Registration
Please fill in the attached pdf registration form and mail it with registration fee to Ms. Vivian Sabiniano at the address below. Also please fax a copy to the number below. This way we will be sure to expect your registration in the mail. Checks should be made payable to
"University of Washington Department of Medicinal Chemistry"
The registration fee covers a light meal at the Tuesday evening reception; light breakfast on Wednesday, Thursday and Friday; lunch and dinner on Wednesday and Thursday.
Also, because this is such a small meeting we would like all who register to submit a title and abstract. This should be sent to [email address removed]. Thanks! These abstracts will be used to select some of the other speaker slots.
If you have any trouble registering, please contact us here:
Email address: [email address removed]
For those wishing to wire funds please follow the instructions "Wire Transfer Requirements for Payor" on the University of Washington's website.
Because we are charged $25 to process this we must pass this charge on to you. Therefore please add $25 to your registration fee. Unfortunately, we cannot accept credit card payment.
Lodging
UPDATE 7 September 2005: Note that the hotel has now informed us that it requires that all who wish to receive the reduced room rate of $165/night must do so through Medicinal Chemistry who are hosting the meeting. Thus at the time you send in your conference registration please indicate arrival and departure dates for nights you will stay at the lodge. We will pass this on to the lodge and confirm with you.
Salish Lodge and Spa is a full service spa modeled after Japanese spas. We picked this location for several reasons:
- Its secluded locale
- Proximity to the airport (45'), Seattle (45') & skiing (30')
- Oddly, because they allow smoking outdoors. Many of the other locations we looked at did not allow smoking anywhere on site.
Each participant is responsible for making their own room reservation with the Lodge. We will, of course, cross check our registration list with the Lodge's registration. Note that there are a limited number of rooms with two beds available. Because the Lodge has only ~ 80 rooms and we would like to accommodate as many as possible, please only book a double bed room if you know you have a room mate. All rooms are available at the same rate of $165/night. So you will save some money with a roommate. Note that each room is quite luxurious and includes a fireplace, Jacuzzi tub and, in the singles, a massive King sized bed. If the Lodge runs out of rooms then please let us know and we will try to find a roommate of your choosing or help you find a hotel near the site. Those not staying at the Lodge will need a car to go back and forth.
Sponsors
We thank each of the following organizations who have provided monetary sponsorship for the meeting. Without them the meeting would not be possible.
- UW School of Pharmacy Mass Spectrometry Facility
- NIAID's WWAMI RCE for biodefense
- NIEHS' UW CEEH
- ThermoElectron
- BrukerDaltonics
- IonSpec
The final program also thanked UW Department of Medicinal Chemistry, Hitachi, Advion and Amgen.
Final program
Tuesday 6 December
Conference Registration, Room Check-in
Lobby
09:00–19:00
Symposium Welcome
David Goodlett, University of Washington, USA · Falls Terrace
19:30–19:45
“The odd electrons are back - and life is exciting again!”
Cathy Costello, Boston University, USA · Keynote Address · Falls Terrace
19:45–20:30
Reception Social and Hors d'oeuvres
Falls Terrace
19:00–23:00
Casual Gathering
Hospitality Suite
from 23:00
Wednesday 7 December
Breakfast
Salish Ballroom
08:00–10:45
Morning Presentations and Discussions · Moderator: Evan Williams, University of California at Berkeley, USA · Salish Ballroom
“New Mass Spectrometry Technology and Applications in the Study of Cell Migration, the Histone Code, and Cancer Vaccine Development”
Donald Hunt, University of Virginia, USA · Salish Ballroom
09:00–09:45
“Towards an Understanding of Electron Transfer Dissociation”
Sharon Pitteri, Purdue University, USA · Salish Ballroom
09:45–10:15
“Electron Capture Dissociation in a linear RFQ ion trap”
Takashi Baba, Central Research Laboratory, Hitachi, Ltd. Japan · Salish Ballroom
10:15–10:45
Break
Salish Ballroom
10:45–11:00
Morning Presentations and Discussions · Moderator: Evan Williams, University of California at Berkeley, USA · Salish Ballroom
“Charge-reduced arginine, lysine, and histidine residues. Structures and energetics”
Frank Turecek, University of Washington, USA · Salish Ballroom
11:00–11:30
“The Electron Transfer Event in Electron Transfer Dissociation”
Jack Simons, University of Utah, USA · Salish Ballroom
11:30–12:00
“Simulations on supercomputer ion dynamics in FT ICR cell in the presence of electrons on the cell axis (ECD case)”
Evgenij Nikolaev, Institute for Biochemical Physics Russian Academy of Sciences, Russia · Salish Ballroom
12:00–12:30
“The Role of Free Radical Rearrangements in Electron Capture Dissociation”
Peter O'Connor, Boston University School of Medicine, USA · Salish Ballroom
12:30–13:00
Lunch
Salish Ballroom
13:00–14:00
Afternoon Presentations and Discussions · Moderator: Frank Turecek, University of Washington, USA · Salish Ballroom
“Effects of Molecular Conformation on ECD”
Evan Williams, University of California at Berkeley, USA · Salish Ballroom
14:00–14:30
“Charge Localization and Backbone Bond Strength Modulation in Peptide Cations Studied by Electron Capture Dissociation”
Frank Kjeldsen, Uppsala University, Sweden · Salish Ballroom
14:30–15:00
“Evolution and revolution in ECD FT-ICR MS implementation”
Yury Tsybin, National High Magnetic Field Laboratory, USA · Salish Ballroom
15:00–15:30
“Predicting fragment abundances in ECD: from full understanding to total confusion and back”
Roman Zubarev, Uppsala University, Sweden · Salish Ballroom
15:30–16:00
Break
Falls Terrace
16:00–16:30
Afternoon Presentations and Discussions Cont’d · Moderator: David Goodlett, University of Washington, USA · Falls Terrace
“Combination of ergodic and non-ergodic ion activation, a new approach for PTM-peptide Identification”
Catherine Stacey, Bruker Daltonics Inc, USA · Falls Terrace
16:30–17:00
“nanoLC Fraction Analysis by Chip-Based Nanoelectrospray for Improved Glycopeptide Characterization”
Gary Williams, Advion BioSystems · Falls Terrace
17:00–17:30
“Finnigan LTQ FT; ECD and Progress”
Stevan Horning, Thermo Electron · Falls Terrace
17:30–18:00
Break
18:00–18:30
Poster Session (Vintage Room) and Beer Tasting
Sponsored by Advion · Falls Terrace
18:30–21:00
Hors d'oeuvres
Falls Terrace
19:00–23:00
Casual Gathering
Hospitality Suite
from 23:00
Thursday 8 December
Breakfast
Salish Ballroom
08:30–11:00
Morning Presentations and Discussions · Moderator: Jon Amster, University of Georgia, USA · Salish Ballroom
“Bioconjugates for Tunable Peptide Fragmentation: Free Radical Initiated Peptide Sequencing (FRIPS)”
Jack Beauchamp, California Institute of Technology, USA · Salish Ballroom
10:00–10:30
“Electron Capture Dissociation of Peptides Metalated with Alkaline Earth Metal Ions”
Dominic Chan, The Chinese University of Hong Kong, Hong Kong · Salish Ballroom
10:30–11:00
“Electron-Promoted Ion Coherence -Enhancement of ECD and LC/MS Sensitivity”
James Bruce, Washington State University, USA · Salish Ballroom
11:00–11:30
“Disruption of non-covalent bonds in large multimeric protein complexes with ECD”
Ron Heeren, Utrecht University, Netherlands · Salish Ballroom
11:30–12:00
Lunch
Salish Ballroom
12:00–13:00
Afternoon Presentations and Discussions · Roman Zubarev, Uppsala University, Sweden · Salish Ballroom
[To Be Announced]
Jasna Peter-Katalinic, University of Münster, Germany · Salish Ballroom
13:00–13:30
“Electron capture dissociation FT-ICR mass spectrometry of proteins involved in signal transduction”
Helen Cooper, University of Birmingham, UK · Salish Ballroom
13:30–14:00
“ECD of Sulfated Glycosaminoglycans - A Tool for Determining the Sites of Sulfation”
Jon Amster, University of Georgia, USA · Salish Ballroom
14:00–14:30
“Electron Capture Dissociation of Triply Charged Peptides Carrying a Proton and a Divalent Metal Cation”
Kristina Hakansson, University of Michigan, USA · Salish Ballroom
14:30–15:00
Break
Vintage Room
15:00–15:30
Poster Session
Vintage Room
15:30–18:00
Break
18:00–19:00
Dinner and Social
Falls Terrace
19:00–23:00
“ECD - Controversy Helps Its Future”
Closing Remarks · Fred McLafferty, Cornell University, USA · Falls Terrace
Casual Gathering
Hospitality Suite
from 23:00
Abstracts and attendees
The abstracts of the talks and posters and the list of meeting attendees are available here.